Figure 3: Inhibition of niclosamide on migration of DU145 cells is STAT3- dependent. A: Blocking STAT3 decreases inhibitory effects of niclosamide on DU145 cell migration. DU145 cells were transfected with STAT3 siRNA or scrambled siRNA (Scr-SiRNA) and subjected to the wound healing assay. Cells were treated with 2 μM of niclosamide, and images were taken at indicated time points after the wounds were created. B: Efficiency of siRNA interference shown by Western blot analysis. Cells were treated as in A and collected for Western blot analysis at indicated time points. C: Quantification of A. The results are representative of three independent experiments.