Figure 4: Inhibition of niclosamide on migration of DU145 cells is mediated by the MAPK signaling pathway. A: Elongated treatment with Niclosamide suppresses the MAPK pathway. DU145 cells were treated with niclosamide at the indicated concentrations for 4 hours (left panel) or 24 hours (right panel) and then collected for Western blot analysis. B: Blocking the MAPK pathway weakens the effect of niclosamide on the migration of DU145 cells. DU145 cells were treated with 2 μM of niclosamide, 2 μM of niclosamide combined with 10 μM of PD98059, or vehicle control. Cells were then subjected to wound healing assay. Images were taken at the indicated time points after the wounds were created. C: Quantification of B. The results are representative of three independent experiments.