Figure 5: Cleavage of pBR322 DNA by complexes 2 and 3 (15-60 μM) in the absence and presence of H2O2 (30 mM) in buffer B (pH 7.2). lane 1, DNA control; lane 2, DNA + H2O2; lane 3, DNA + 2 (15 μM) + H2O2; lane 4, DNA + 2 (30 μM) + H2O2; lane 5, DNA + 2 (60 μM) + H2O2; lane 6, DNA + 2 (30 μM) + H2O2+ L-Histidine (0.02 M); lane 7, DNA + 2 (30 μM) + H2O2+ DMSO (2 μL); lane 8, DNA + 3 (15 μM) + H2O2; lane 9, DNA + 3 (30 μM) + H2O2; lane 10, DNA + 3 (60 μM) + H2O2; lane 11, DNA + 3 (30 μM) + H2O2+ L-Histidine (0.02 M); lane 12, DNA + 3 (30 μM) + H2O2+ DMSO (2 μL).