Figure 3: DNJ dynamics during the different developmental stages of the silkworm. Note: 20 samples were taken from different developmental stages and numbered as follow: 1. Newly hatched larvae; 2. Molting larvae of instar I; 3. Molted larvae of instar II; 4. Molting larva of instar II; 5. Molted larva of the instar III; 6. Molting larvae of instar III; 7. Molted larvae of instar IV; 8. Molting larvae of instar IV; 9. Molted larvae of instar V; 10. Day 1 of instar V; 11. Day 2 of instar V; 12. Day 3 of instar V; 13. Day 4 of instar V; 14. Day 5 of instar V; 15. Day 6 of instar V; 16. Matured larvae; 17. Day 1 of cocooning; 18. Day 2 of cocooning; 19. Pupae; 20. Moth. Values are expressed as the mean ± S.D. (n=3). Means without the same letters were considered significant , tested by one-way ANOVA and Fisher’s LSD, p ≤ 0.05.