NO. Sample Time NO. Sample Time 1 newly-hatched larva Day 1 11 Day 2 of instar V Day 17 2 molting larva of instar I Day 3 12 Day 3 of instar V Day 18 3 molted larva of instar II Day 4 13 Day 4 of instar V Day 19 4 molting larva of instar II Day 6 14 Day 5 of instar V Day 20 5 molted larva of instar III Day 7 15 Day of instar V Day 21 6 molting larva of instar III Day 10 16 matured larva V Day 22 7 molted larva of instar IV Day 11 17 Day 1 of cocooning Day 23 8 molting larva of instar IV Day 15 18 Day 2 of cocooning Day 24 9 molted larva of instar V Day 15+ 19 pupa Day 26 10 Day 1 of instar V Day 16 20 moth (adult) Day 36

Note: “Sample” means the physiological stage of the silkworm, while “Time” means the corresponding time point during the whole life cycle of silkworm. No. 8 and No. 9 were collected at 8:00 and 18:00 of the same day, respectively.

Table 1: Silkworm samples and corresponding developmental stages.