10-minute incubation 2-hour incubation
Comp. R n IC50 (mM) SD IC50 (mM) SD
4 H 1 6.79 0.12 1.58 0.54
5 CH3 1 3.72 0.26 0.78 0.07
9 H 0 8.85 1.67 2.59 0.51
10 CH3 0 12.01 0.13 2.12 0.16
Diltiazem 0.26* 0.06 0.14 0.02
Verapamil 0.45** 0.12 0.12 0.11
Experiments were repeated for four times for each compound at each incubation> time tested.
*Reported 0.30 μM [20], ** Reported 0.47 μM [20].
Table 1: IC50 (μM) values of hybrid compounds, diltiazem and verapamil after 10-minute and 2-hour incubation times