Analyte Spiked conc. In bulk In serum
(µg mL-1) % Rec. % RSD % Rec. % RSD
Prazosin 8 100.08 0.009 100.04 0.004
10 99.93 0.004 99.97 0.002
12 100.65 0.001 100.07 0.008
Amlodipine 32 100.21 0.008 100 0.018
40 100.09 0.085 100.03 0.008
48 99.55 0.002 99.98 0.001
Diltiazem 16 100.17 0.007 100.06 0.011
20 100.09 0.005 99.96 0.005
24 100.1 0.001 100.03 0.002
Verapamil 48 100.05 0.002 100.05 0.004
60 99.94 0.003 99.92 0.004
72 99.98 0.054 100.09 0.017
Table 3: Accuracy of the proposed method