Parameters   % RSD
Chromatographic Condition Variation PRZ AML DIL VER
Mobile phase composition MeOH:H2O:ACN (50:40:10) 0.047 0.011 0.015 0.057
MeOH:H2O:CAN (60:30:10) 0.01 0.017 0.019 0.021
pH of mobile phase 2.63 0.745 0.042 0.054 0.145
2.67 0.014 0.019 0.014 0.021
Flow rate (mL min-1) 0.9 0.241 0.032 0.061 0.01
1.1 0.417 0.421 0.019 0.005
Column temperature (°C) 22 0.528 0.247 0.013 0.007
30 0.015 0.087 0.041 0.018
Detection wavelength (nm) 238 0.065 0.024 0.081 0.045
242 0.021 0.004 0.014 0.085
Table 5: Robustness of the method