Figure 2: TBQ-induced apoptosis is associated with caspase activation and decreased expression of anti-apoptotic proteins in CML cells. (A) TBQ induced PARP cleavage and caspase activation in KBM5 and KBM5-T315I cells. Cells were dose- and time-dependently treated with TBQ. PARP and caspase-3, -8 were analyzed by western blots. GAPDH was used as a loading control. C: control. The quantitative analyses of western blot results of PARP are summarized in the bar graphs accompanying the representative images, Mean ± SD (n=3). (B) TBQ decreased the expression of anti-apoptotic proteins in KBM5 and KBM5-T315I cells. Cells were dose- and time-dependently treated with TBQ. The anti-apoptotic proteins Bcl-2, Mcl-1 and XIAP were analyzed by western blot.