(A) TBQ induced down-regulation of Bcr-Abl and its downstream signaling proteins. KBM5 and KBM5-T315I cells were dose- and time-dependently treated with TBQ. Bcr-Abl and its downstream proteins were detected by western blot analyses. GAPDH was used as a loading control. C: control. (B) Caspase inhibition blocked TBQ from decreasing Bcr-Abl and its downstream signaling proteins. KBM5-T315I cells were treated with 7.5 μM TBQ with/ without caspase inhibitor z-VAD-fmk (20 μM) for 12 hours. The total and phosphorylated Bcr-Abl and its downstream proteins were detected by western blot analyses. The quantitative analyses of the western blot results are summarized in the accompanying bar graph, Mean ± SD (n=3).