R1 R2 R3 ΔTm (°C) 5 H H 3-Cl-4-NHCOOC2H5 0 24 NH2 H 3-Cl-4-NHCOOC2H5 8.1 ± 0.8 25 NH2 CH3 3-Cl-4-NHCOOC2H5 6.3 ± 0.6 EBE-A22 H CH3 3-Br 8.1 ± 0.8

Variations in melting temperature (ΔTm=TmDrug-DNAcomplex– TmDNAalone) at drug/ctDNA ratio of 1. Means values are calculated from at least two separate experiments with typical standard deviations of less than ± 10%.

Table 3: DNA thermal denaturation results.