Compound Trypanocidal activity (%)a   Epimastigote Trypomastigote Amastigote 10 μM 100 μM 10 μM 100 μM 10 μM 100 μM 2 NA 10 ± 1 12 ± 1 34 ± 1 - - 3 NA 11 ± 1 27 ± 1 37 ± 2 - - 4 12 ± 1 29 ± 2 11 ± 1 53 ± 4 - - 5 15 ± 1 78 ± 6 71 ± 6 96 ± 6 73 ± 7 100 ± 4 Nifurtimox 52 ± 2 100 ± 3 36 ± 3 100 ± 2 45 ± 3 100 ± 3 Values (mean ± SD) were obtained from three independent experiments. NA inactive at concentration tested. aResults are reported as the percentage of non-viable parasites regarding the control at 10 and 100 μM. - Not tested

Table 1: Trypanocidal activity in different parasite stages for compounds 2-5 and the reference compound Nifurtimox at two different concentrations.