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Figure 1: (A) Elution profile of Bothriopsis taeniata venom by molecular exclusion chromatography on a Sephadex G-75 column (Kontex Flex Column 78x2 cm). Fraction III contained PLA2 activity. (B). Elution profile of peak III following RP-HPLC on a reversed-phase column, shim-pack CLC-ODS (C18), 4.6 mmx25 cmx0.5 μm. The peak corresponding to the phospholipase A2 (Btae TX-I) from Bothriopsis taeniata venom is indicated (*). |