Figure 6: (A) Twitch-tension responses of indirectly stimulated chick biventer cervicis preparation. The preparations were incubated with Btae TX-I PLA2 (5–50 μg) active fraction, for 120 min at 37°C. (B) Representatives myographic profiles showing the muscle response to indirectly stimulated (0.1 Hz, 0.2 ms) muscle preparation and contractures in response to exogenous ACh (●) and KCl (■). Btae TX-I in different concentrations were added at t =0 and left in contact with the tissue for the duration of the experiment. Each point represents the average from five experiments ± SEM; p < 0.05 compared with control.