Figure 2: Cytotoxicity and ROS production in human lung cells: Cytotoxicity, BEAS-2B, WI-38-VA13, and A549 cells were cultured in 96-well plates and treated with SWCNTs from 0 to 200 μg/ml for 1 to 6 days. Cell proliferation/cell death was detected with CellTiter 96 Aqueous One Solution Cell Proliferation Assay regents. Data represent means ± SD from three samples. D. ROS production. A549, BEAS-2B, WI38-VA13 cells were cultured in 8-well chamber slides and treated with 20 μg/ml SWCNTs for 16 h. The ROS detection fluorescence probe DHE was added 30 min prior to the end of treatment. The nucleus was stained with DAPI. Image was taken using a confocal fluorescence microscope.