Figure 1: Effects of THC on MBP immunostaining. Aggregate cultures remained either untreated (A), or were treated with THC (1 μM) (B); IFN-γ (50 U/ml) plus LPS (5 μg/ml) (C); or treated with THC simultaneously with the inflammatory agents (D). Cultures were harvested 48 hours after the last treatment with the inflammatory agents. The MBP staining was quantified (E), measuring 20 aggregate sections per treatment and expressing the stained area as percent of untreated control cultures. The Figure shows representative data from one experiment. Results were statistically evaluated for significance by one-way ANOVA test followed by the Tukey post-test. (*P<0.05, ***P<0.001, compared with untreated control cultures; °°°P<0.001 compared with cultures treated with the inflammatory agents). Bar = 100μm.