Figure 2: Effect of NEM and buterol on the coulping of mitochondria. Effect of buterol (A) and NEM (B) on changes in ΔΨm induced by 50 μM ADP. ΔΨm was estimated by a TPP+-selective electrode in a medium containing 120 mM KCl, 10 mM HEPES, 1.5 mM phosphate, pH 7.25, and 4 mM succinate. Both compounds increase the time of phosphorylation and decrease the degree of the coupling. The curves are typical for five identical experiments.