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Figure 4: Pattern RAPD-PCR of DNA samples treated with the pharmacological substances. RAPD-PCR fingerprinting of sperm generated by primer D11(a) and F1(b) incubated with the tested drugs for 15- 30-45 minutes. The appearance and disappearance of bands and intensity variations of the same pattern than the negative control were considered. M = marker 100 bp; N = control. For drugs abbreviations see Figure1. |