Sr. No. Dilution Factor Concentration (ppm) Total animals Mortality Mortality percentage
1 0 0 10 0 0%
2 200 5 10 0 0%
3 100 10 10 2 20%
4 50 20 10 5 50%
5 33.33 30 10 6 60%
6 20 50 10 7 70%
After 96 hours exposure to the effluent sample, in 10ppm concentration there was 20% mortality, in 20ppm there was 50% mortality, in 30ppm concentration there was 60% mortality and in 50ppm concentration there was 70% mortality
Table 5: 96 Hours Mortality.