True [Ng/Ml]

Measured [Ng/Ml]

Diff %

2.95

2.91

1.37

3.61

3.6

0.28

1.22

1.2

1.67

0.454

0.437

3.89

0.15

0.154

-2.60

0.38

0.385

-1.30

1.63

1.51

7.95

4.35

4.52

-3.76

6.89

6.76

1.92

0.348

0.34

2.35

0.354

0.389

-9.00

10.000

10.6

-5.66

0.360

0.336

7.14

2.200

2.14

2.80

0.083

0.085

-2.35

7.610

7.58

0.40

2.130

2.140

-0.47

1.470

1.390

5.76

2.440

2.430

0.41

0.891

0.879

1.37

0.778

0.757

2.77

Table 1:Nanoparticle pre-processing step passed the concentration method verification test in our laboratory, N=21. TRUE = true value of HGH concentration in the standard sample, MEASURED = HGH concentration measured in the standard sample in a blind fashion, DIFF% = (MEASURED - TRUE)/TRUE *100. Acceptability criteria = DIFF% < 10%.