Figure 7: Representative sphere formation from passage 6 (P6) cultured human corneal endothelial cells (CECs). (A) P6 cultured CECs derived from a 43-year-old donor were grown as single cells in serum-free floating culture for 7 days. Dissociated cells are observed in medium containing methylcellulose gel matrix. (B) A sphere colony has grown from a single cell on day 3 (C) and has become larger on day 7. (D) Adherent progeny that migrated from the sphere colony (E). Scale bars=100 μm.