Figure 2: Gene expression of TNF-a, TACE/ADAM-17, and GAPDH in liver tissue of TIMP-3-treated rats compared to saline-treated rats exposed to I/R injury. Total mRNA extracted from rat liver tissues were analyzed by RTPCR for TNF-a, TACE/ADAM-17, and GAPDH mRNA. TIMP-3-treatment and saline treatment are denoted by + and -, respectively. All rats underwent 30 minute ischemia, followed by varying reperfusion duration (6 hrs, 24 hrs, 48 hrs or 7 days). The PCR products were analyzed by agarose gel eletrophoresis, stained with ethidium bromide.