Solvent system and ratio Rf value
Petroleum ether: Ethyl acetate (1:2) 0.73
Chloroform: Ethyl acetate (49:1) 0.92
Ethyl acetate: Chloroform (19:1) 0.79
Toluene: Ethyl acetateĀ  (3:1) 0.88
Ethyl acetate: Pyridine: Water (5:1:4) 0.98
Ethyl acetate: Chloroform: Methanol (2:2:1) 0.95
Benzene : Ethyl acetate (19:1) 0.86
Chloroform: Ethyl acetate (4:1) 0.91
Ethyl acetate: Acetone (9:1) 0.94
Table 3: TLC analysis of the compound on silica gel G.