| |
BALF Cytokine Concentration (pg/ml) |
| |
Time |
1 hour |
2 hour |
4 hour |
8 hour |
24 hour |
| IL-6 |
IKK-2 KO |
519 ± 37 |
2316 ± 325 |
3698 ± 728** |
1500 ± 437 |
28 ± 16 |
| |
Control |
1005 ± 215 |
1532 ± 216 |
1984 ± 216 |
859 ± 142 |
16 ± 14 |
| IL-10 |
IKK-2 KO |
55 ± 10 |
142 ± 18 |
138 ± 24* |
36 ± 20 |
8 ± 4 |
| |
Control |
124 ± 16 |
133 ± 11 |
84 ± 19 |
28 ± 10 |
18 ± 6 |
| IL-12 |
IKK-2 KO |
0 ± 0 |
159 ± 44 |
223 ± 33 |
46 ± 41 |
0 ± 0 |
| |
Control |
99 ± 16 |
244 ± 15 |
317 ± 170 |
38 ± 12 |
29 ± 20 |
| KC |
IKK-2 KO |
670 ± 88** |
1026 ± 185 |
731 ± 86 |
85 ± 26 |
17 ± 9 |
| |
Control |
1204 ± 126 |
1275 ± 305 |
912 ± 214 |
149 ± 13 |
68 ± 13 |
| TNF-α |
IKK-2 KO |
1433 ± 127 |
1340 ± 369* |
547 ± 185 |
53 ± 10 |
12 ± 2 |
| |
Control |
1249 ± 186 |
720 ± 364 |
360 ± 114 |
63 ± 19 |
12 ± 3 |
Mice were challenged with aerosolised LPS and killed at various time points post-challenge. Lungs were lavaged and cytokine levels in BALF assayed. Values are
geometric mean ± SEM of 4 animals. *P<0.05, **P<0.005 (knockout animals vs controls) |
