Data is represented as mean fold change from untreated at each respective time point ± SEM. Letter a indicates p< 0.05 for all challenges vs. untreated at each respective time point.
|
| Figure 6: Effects of repeated O2, IR and combined challenge on Nitrotyrosine
formation, during the late phase post termination of the challenge exposure.
Separate cohorts of mice were exposed to 100% O2 for 8 hours only (O2), 0.25
Gy total body ionizing gamma irradiation (IR) only, or a double-hit combination
of both challenges (O2+IR) followed by intermittent normoxia (21% pO2) for
repeated cycles 3 times a week for 4 weeks. Mice were sacrificed at 20 weeks
(chronic). Panels A-D Representative sections from paraffin lung sections
stained for nitrotyrosine (brown staining) from untreated (A), O2 (B), IR (C)
and O2+IR (D) animals. Sections were counterstained with crystal blue. Panel
E Representative western blot validation of NT formation in lung homogenates
from the same mouse cohorts as above. Panel F Densitometric analysis with
beta actin normalization of the combined levels of all 3 major NT bands for
each cohort. Data is represented as mean fold change from untreated at each respective time point ± SEM. Letter a indicates p< 0.05 for all challenges vs. untreated at each respective time point. |
