| Mouse Bodyweight (g) |
| Time Point (Week) |
| |
0 |
1 |
2 |
4 |
8 |
12 |
16 |
20 |
| Untreated |
19.2 ± 0.1 |
19.6 ± 0.1 |
20.0 ± 0.1 |
21.7 ± 0.0 |
23.8 ± 0.3 |
25.7 ± 0.2 |
29.1 ± 0.2 |
30.2 |
| O2 |
19.4 ± 0.3 |
19.6 ± 0.2 |
19.8 ± 0.2 |
21.2 ± 0.3 |
22.6 ± 0.3a |
24.9 ± 0.4 |
27.1 ± 1.1 |
31.3 |
| IR |
18.8 ± 0.7 |
19.0 ± 0.7 |
19.3 ± 0.5 |
20.3 ± 0.3a |
22.8 ± 0.4a |
26.1 ± 1.1 |
30.3 ± 5.1 |
37.5 |
| O2 + IR |
18.8 ± 0.5 |
19.0 ± 0.5 |
19.1 ± 0.4 |
20.1 ± 0.3a |
21.9 ± 0.3a |
24.1 ± 1.3 |
29.6 ± 1.0 |
33.5 |
ap<0.05 for all challenges versus untreated at respective time point Values reported as mean ± standard error of the mean
Separate cohorts of mice (n=5-15 / group) were exposed to 100% O2 for 8 hours only (O2), 0.25 Gy total body ionizing gamma irradiation (IR) only, or a double-hit
combination of both challenges (O2+IR) followed by intermittent normoxia (21% pO2) for repeated cycles 3 times a week for 4 weeks. Mice were sacrificed at weeks
1, 2, and 4 weeks (acute) and 8, 12, 16, and 20 weeks (chronic). (Panel A) Body weight was recorded weekly throughout the course of the study. At 4 and 8 weeks,
mouse cohorts exposed to challenge conditions had significantly lower bodyweight compared to untreated mice at respective time points. Letter a indicates p< 0.05 for all
challenges vs. untreated. |
