Figure 4: Lung leak caused by VEGF. Uninjured isolated perfused rat lungs (IPRL) were studied with 125I-albumin added to the perfusate to determine the lung vascular leak caused by unlabeled VEGF (250 pM, or 5,750 pg/ml) compared to PBS vehicle. 125IVEGF was not used in these experiments. Leak was determined by counts per minute (CPM) of radioactivity per gram of wet lung tissue. Vascular leak with unlabeled VEGF was 1.66 ± 0.21 times that seen with PBS vehicle (mean ± SEM). Perfusion with 125Ialbumin was done for 15 minutes after VEGF addition. * P < 0.05 by paired t-test