Figure 2: Detection of Mammalian Lignans in Mouse Plasma.Separate cohorts of mice (n=5/group) were fed 0% or 10% FS diet and exposed to 100% O2 for 8 hours only (O2), 0.25 Gy total body ionizing gamma irradiation (IR) only, or a double-hit combination of both challenges (O2+IR)followed by intermittent normoxia (21% pO2) for repeated cycles 3 times a week for 2 weeks. Mice were sacrificed after 2 weeks of challenge exposure and plasma analyzed for levels of circulating mammalian lignans enterodiol(Panel A) and enterolactone (Panel B) using GC/MS/MS. Data is represented as mean ± SEM.