Figure 3: Flaxseed ameliorates increased Hyperoxia, Radiation, or Double-Hit Combination Challenge Induced Lung Inflammation and Proinflammatory Cytokine Release.Separate cohorts of mice (n=5/group) were fed 0% or 10% FS diet and exposed to 100% O2 for 8 hours only (O2), 0.25 Gy total body ionizing gamma irradiation (IR) only, or a double-hit combination of both challenges (O2+IR)followed by intermittent normoxia (21% pO2) for repeated cycles 3 times a week for 2 weeks. Mice were sacrificed after 2 weeks of challenge exposure. BALF was evaluated for total neutrophil cell counts (Panel A) and levels of pro-inflammatory cytokines, IL-1β (Panel B) and TNFα (Panel C). Data is represented as mean ± SEM. *p< 0.05 for O2, IR, and O2+IR versus respective untreated control mice in each diet cohort. #p< 0.05 for respective 0% FS fed mice exposed to O2, IR, and O2+IR versus 10% FS fed mice exposed to O2,IR, and O2+IR.