Figure 2: Western immunoblots with pure recombinant proteins and rabbit sera obtained after second booster from animals immunized with pure PE35 (panel a), PPE68 (panel b), ESXA (panel c), ESXB (panel d) and ESXV (panel e). M: Prestained low molecular weight marker. The arrow in each panel shows the band for antibody reactivity with the specific protein that was used for immunization, i.e. panel a, lane 1: PE35; panel b, lane 5: PPE68; panel c, lane 2: ESXA, panel d, lane 1: ESXB, panel e, lane 5: ESXV. The other lanes in each panel were loaded with other proteins.