Figure 5: Western immunoblot analysis of proteins present in the culture filtrate (MT-CF) and cell wall (MT-CW) preparations of M. tuberculosis after probing with sera obtained from rabbits immunized with pure recombinant PE35 (a), PPE68 (b), ESXA (c), ESXB (d), and ESXV (e). M: Prestained low molecular weight marker. Lane 1: MT-CF, Lane 2: MT-CW. The arrows indicate regions of antibody reactivity.