Figure 3: Production of IFN-? by cell suspensions from different organs stimulated with different mycobacterial antigens after vaccination and before challenge: Mice were vaccinated with the mutant mce-2A or BCG bacteria and after 15, 30 and 60 days the indicated organs were used to get cell suspensions which were stimulated with whole culture filtrate antigens (CFA) or the immunodominant antigens ESAT-6 or Ag85, IFN? was quantified by ELISA in the supernatants. Bars represent the means and standard deviations of four different animals at each time point. Asterisks represent statistical significance (P< 0.05) among the indicated groups.