Figure 1: Protein localization in mycobacteria using the agarose embedding based IF method. Monoclonal antibodies were used to stain the antigens Ag84 and Ag85B in M. tuberculosis H37Rv (A) and MUL2232 and MUL3720 in M. ulcerans NM20/02 (B) in formalin fixed agarose embedded paraffin sections. For MUL2232 / MUL3720 double staining (C), IgG subclass specific secondary antibodies were used. Scale bar: 2 μm.