Sample Location Culture Medium Fungi CFU/Swab Culture Medium BacteriaCFU/Swab
Vent–Top MEA 840,000 TSA 3,000,000
Vent - Bottom MEA 1,100,000 TSA 60,000
AHU – Bottom Front MEA 310,000 TSA 110,000
AHU- Bottom Center MEA 490,000 TSA 3,100,000
AHU- Bottom Back MEA 130,000 TSA 100,000
AHU – Floor on Left MEA 2,500,000 TSA 3,00,000
Blackened Paper – Floor MEA 1,200,000 TSA 31,000,000
AHU = Air Handling Unit
Most Common Fungi: Aspergillus niger, Aureobasidium pullulans, Mucor plumbeus, Rhizopus stolonifer, Rhodotorula mucilagenous, Cladosporium, Penicillium spp, Trichoderma hazarianumm, Cunningham elegans, yeast.
Most Common Bacteria: Bacillus spp, Gram negative rods, Gram positive cocci.
Non-Viable Spores: Non-viable spore samples were taken from a wall cavity with a telephone jack: Penicillium/Aspergillus; Cladosporium and Stachybotrys chartarum.
Q-PCR: ERMI tests: ERMI tests were performed on two samples. Identified fungi were: following: Acremonium strictum, Aspergillus fumigatus, penicillioides, niger, unguis, and ustus, Aureobasidium pullulans, Cladosporium herbarium, Eurotium amstelodami, Mucor/Rhizopus, Paecilomyces variotii, Penicillium chrysogenum, purpurogenum, spinulosum and variable, Rhizopus stolonifer and Wallemia sebi
Wall Cavities: Wall check samples at two outlets (electrical and phone jack) collected by Air-0-Cell cassettes (14 L/min for 5 minutes) detected Aspergillus/ Penicillium at 95 and 87.5 % of the total spore counts of 390 and 1,600, respectively. Stachybotrys spores were detected in the phone jack outlet at 0.008 % if the total spore counts.
Table 1: This table summarizes the Fungi and bacteria identified in the building from cultures of swab samples obtained from patient’s office. All areas were contaminated with fungi and bacteria.