Glucose tolerance tests were performed on control wild type (WT) and miR- 150 knockout (miR-150-KO) mice fed with high fat diet for 16 weeks at age of 20 weeks. Experiments were performed in fasting overnight male mice. Glucose solutions were injected into peritoneal cavity at the dose of 1.0 ml/kg (1M glucose solution). Blood was collected via tail vein at the indicated time points. Glucose concentration in plasma was measured using a glucometer (Precision); n=10 in each group of mice. Insulin tolerance tests were performed on control wild type and miR-150 knockout mice under high fat diet condition for 18 weeks at age of 22 weeks. Experiments were conducted similar as the described above for the glucose tolerance tests but fasting 6 hours before the injections and insulin solutions were injected into peritoneal cavity at the dose of 0.5 U/kg body weight. Blood was collected via tail vein at the indicated time points, and glucose levels were measured; n=10 in each group of animals, Error bars represented the ± SEM, *p<0.05 and **p<0.01.
