Figure 2: Dot-blot immundetection of polysacharides A. Dot blot of commercially available polysaccharides used as controls. Zero to 1 μg of xyloglucan (tamarin seeds), xylans (beechwood), guar and pectins (citrus) were solubilized in water (upper lane) or in IL (lower lane) and spotted onto nitrocellulose membranes before being revealed with LM15, LM10, LM21 and JIM5 mAbs. Two μg of polysaccharides were spotted for LM10 and LM21 and 20 μg for LM15 and JIM. B. Dot blot analysis of the walnut wood polysaccharides fractionnated according to Vanzin et al. [3] (same mAbs). Upper line: B to E enriched fractions containing pectins; F1 and F4 enriched fractions containing hemicelluloses. Two μg of polysaccharides were spotted for LM10 and LM21 and 20 μg for LM15 and JIM C. IL solubilized walnut wood polysaccharides according to protocols I, II, III and IV. In each column, 0.05 to 1 μl of crude solution were diluted in 200 μl of water, spotted and detected with LM15, LM10, LM21 and JIM5 mAbs.