Figure 4: Vero cells infected with amastigotes of T. cruzi for 36 h and stained with Acridine orange. Untreated cells (a and b) and treated with compound 1 at 1 mM for 12 h (c and d). Black arrows show the amastigotes and white arrows the increase of acidification of the cytoplasm. N=nucleus Scale bars: A-D=50 μm.