Figure 5: Therapeutic efficacy of Lip-C6 and Lip-Saf using human patient AML is reflected by a decrease of autophagy. The Cyto-ID Autophagy Detection Kit was used to visualize autophagy by flow cytometry using a favorable-prognosis human AML patient sample characterized as having translocation 8;21 (patient 632). Cells were also stained to analyze the leukemia progenitor population (CD34+CD38-). The combination of Lip-C6 (10 μM) and Lip-Saf (5 μM) dramatically decreased autophagy. Percentages displayed are for cells undergoing the highest level of autophagy. The intermediate stained population, not gated as positive, corresponds to normal lymphocytes.