| Research Article |
Open Access |
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| Pharmacokinetics of Casiopeína IIgly in Beagle Dog: A Copper Based Compound with Antineoplastic Activity |
| Cañas-Alonso RC1, Fuentes-Noriega I1 and Ruiz-Azuara L2* |
1Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México, 04510 México D.F., México.
2Facultad de Química, Departamento de Química Inorgánica y Nuclear, Universidad Nacional Autónoma de México, 04510 México D.F., México |
| *Corresponding author: |
Lena Ruiz-Azuara, Ph.D. Facultad de Química.
Departamento de Química Inorgánica y Nuclear, Universidad Nacional
Autónoma de México, 04510,
Tel/Fax: (5255) 56223529
E-mail: ruizazuara@gmail.com |
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| Received January 27, 2010; Accepted March 22, 2010; Published March 22, 2010 |
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| Citation: Cañas-Alonso RC, Fuentes-Noriega I, Ruiz-Azuara L (2010) Pharmacokinetics of Casiopeína IIgly in Beagle Dog: A Copper Based Compound with Antineoplastic Activity. J Bioanal Biomed 2: 028-034. doi: 10.4172/1948-593X.1000018 |
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| Copyright: © 2010 Cañas-Alonso RC, et al. This is an open-access articldistributed under the terms of the Creative Commons Attribution License, hich permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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| Abstract |
| Casiopeína IIgly is a mixed chelate coordination complex with copper core that have demonstrated high antineoplastic activity in vitro and in vivo. In the present work, a developed and validated method for measurement of Casiopeína IIgly in beagle dog and its application in a pharmacokinetic study is reported. The analyte was isolated from blood by solid-phase extraction using Strata X cartridges. The analysis was carried out on a Synergy Polar-RP column (30 X 2.0 mm) using an isocratic elution and MeOH/ HFBA 0.1% (4:6) as the mobile phase. An Agilent LC/ MSD Trap VL equipped with an ionization electrospray source, was operated in selective ion storage (SIS) usingstable ion [Cu(II)(F7C3COO) 4,7-dimethyl phen ]+ with 484 m/z for quantification result of ESI reaction betweenCasiopeína IIgly and HFBA. The method demonstrated to be linear (r = 0.9992) in the range from the 0.1 to 15 μg/ml
with a limit of detection (LOD) of 50 ng/ml. All the parameters of validation such as selectivity, accuracy, precision and recovery were within the required limits. Pharmacokinetics
assay was carried out at 2 doses, indicated a high elimination rate. |
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