Case No Species H&E Fontana-Masson Bleaching method S-100  protein HMB-45 Tumor location Benign/
Malignant
1 Horse Melanocytoma + + + + Dermal Benign
2 Horse Melanocytoma + + + + Dermal Benign
3 Horse Melanocytoma + + ± ± Dermal Benign
4 Horse Melanocytoma + + ± ± Dermal Benign
5 Horse Melanocytoma + + + + Dermal Benign
6 Horse Melanoma + + ± ± ± Malignant
7 Cattle Melanocytoma + + + + Dermal Benign
8 Cattle Melanocytoma + + + + Dermal Benign
9 Cattle Melanocytoma + + + + Dermal Benign
10 Horse SCC - - - - - Malignant
11 Horse SCC - - - - - Malignant
12 Cattle SCC - - - - - Malignant
13 Cattle SCC - - - - - Malignant
Table 1: Comparative results of melanocytic tumors by H&E, special staining and Immunohistochemical methods.
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