QC Level Predicted
concentrationa (ng/
mL)
Mean
accuracye
(%)
Intra-day repeatability
(%RSD) (n = 5)
Inter-day
repeatability
(%RSD) (n
Meanb ± = 30)
Meanb ±SDc %RSDd Day-1 Day-2 Day-3
LQC
(250 ng/mL)
258.14 ±
10.62
4.11 3.25 4.04
3.78
4.10
3.97
4.06
4.04
4.02
MQC
(750 ng/mL)
740.85 ±
23.14
3.12 -1.22 3.24
3.08
3.17
2.97
3.26
3.14
3.17
HQC
(3000 ng/
mL)
3037.75
± 73.68
2.42 1.25 2.54
2.78
3.17
2.97
2.86
3.04
2.75
aEach value is mean of ten independent determinations (n = 10).
bPredicted concentration of RLZ was calculated from linear regression equation.
cStandard deviation.
dPercentage relative standard deviation.
eAccuracy is given in relative error % = [100 × (predicted concentration – nominal concentration)/nominal concentration.)].
Table 1: Results of accuracy and intermediate precision studies for the proposed method in rat plasma.