Figure 4: CASTp pocket prediction: Heme is indicated as brown sphere shape and Ala 102 Pro mutant position in Red and other pocket residues are indicated as lines with labels. Ala102 was found to structurally orient near the active site along with other heme binding residues: ASP49, HIS51, HIS57, HIS71. Hence the mutation in Ala102Pro shall affect the PZA activity in terms of substrate binding.