SAVS (Structure Analysis and Validation Server) Before Energy Minimization After Energy Minimization WILD MUTANT WILD MUTANT Most favoured regions 84.3% 90.5% 87.3 % 91.9% Additional allowed regions 12.6% 7.0% 12.7 % 8.1% Generously allowed regions 1.3% 1.9% 0% 0% Disallowed regions 1.9% 0% 0% 0% ProQ LG score 5.167 5.340 6.064 5.006 Max sub 0.187 0.228 0.594 0.511 Q mean score - - 0.82 0.79

Table 1: Energy minimization and Protein structure Quality assessment of WT and MT of Pzase.