Position 1 3 6 10 11 13 16 19
G1 effective   A   T   A/C/T   A/T
G2 effective G/C             A/T
ineffective A/T             G/C
G3 effective G/C   A     T C A/T
ineffective T     T       G
G4 effective G/C     A/T       A/T
G5 effective         C/G A G T
ineffective     C   A/T     G
position: nucleotide position from 1 to 19 (5’ to 3’, cDNA form) effective: preferred, ineffective: unpreferred
Table 1: Effective and ineffective nucleotides specified in the individual guidelines.