No. of genes No. of siRNAs Description Technique
Reynolds et al. [18] 2 197 Sequence features  
Ui-Tei et al. [19] 6 72 Sequence features  
Amarzguioui et al. [20] 4 46 Sequence features  
Hsieh et al. [21] 22 138 Sequence features  
Hesken et al. [23] 34 2128 Sequence motifs Neural network
Jagla et al. [22] 4 601 Sequence features Decision tree
Holen [27] 34 400 Sequence features Percentage
Saetrom [28] 40 581 Sequence motifs Genetic programming
Teramoto et al. [14] 2 94 Sequence motifs Support vector Machine
Ladunga [29] 34 2252 Position features Support vector Machine
Calk et al. [13] 92 398 Binding energy Regression tree
Takasaki et al. [30-32] 490 833 Sequence features Statistics, SOM, RBF
Table 2: Features of individual siRNA design rules.