Residues Cnsw (6,8) Cnsw (21,22)Cnie (6,8) Cnie (21,22) Actin binding S83T 0.02403 0.08153 0.01511 0.19407   V87I -0.00468 0.06226 0.00442 0.19407   P161A 0.02264 0.24512 0.00125 0.19407   Q164E 0.03766 0.13165 0.01125 0.19407   E168D 0.00917 0.13241 0.00984 0.19407   T176A 0.00567 0.15339 0.00740 0.19407   D186A -0.00321 0.24408 0.00252 0.19407   A189P -0.00725 0.28690 -0.00221 0.19407   N197H -0.00107 0.25931 0.00426 0.19407   A206P 0.00098 0.05119 0.01027 0.08149   T217E 0.02192 0.23347 0.01228 0.19407   D220E 0.01581 0.03949 -0.00070 0.11018   T226V -0.00024 0.22298 0.00951 0.19407   T227S -0.00233 0.07602 0.00579 0.19407   C234T 0.00033 0.29774 0.00084 0.19407   V271I -0.00281 0.06226 -0.00071 0.19407   K273R -0.00389 0.11880 -0.00113 0.19407   V285I 0.03537 0.06226 0.01110 0.19407   V320I 0.00474 0.06226 -0.00023 0.19407   A326C 0.00771 0.31076 0.00082 0.19407   N331S 0.00691 0.20197 0.00510 0.25547 X M335Q 0.03814 0.27765 0.01750 0.19407 X G339Q -0.02090 0.16000 -0.00380 0.15548   A342S -0.00375 0.10550 -0.00168 0.19407   A358S 0.03212 0.10550 0.01011 0.19407   L361I 0.02974 0.02915 0.01903 0.15548   G390N -0.00206 0.08175 -0.00219 0.11018   F393S 0.01559 0.34059 0.02170 0.19407   D403E 0.00208 0.11476 0.01418 0.19407   D417Q -0.00424 0.19312 -0.00304 0.19407   A440N -0.01732 0.24580 -0.00275 0.19407   D442E 0.00573 0.03949 0.00231 0.11018   A445S -0.00473 0.10550 0.00362 0.19407   A446G -0.00585 0.14825 0.00157 0.11018   K462G -0.02254 0.33389 -0.00195 0.19407   -463K -0.00848 0.23160 -0.00183 0.19407   AUC 0.83824 0.75000 0.77941 0.80147   Threshold 0.00632 0.19754 0.00476 0.22477   Sensitivity 0.67647 0.64709 0.61765 1.00000   Specificity 1.00000 1.00000 1.00000 0.50000

Table 1: Four conservation score sets with the first and second highest AUC in 378 (=28C2) conservation score sets of both variabilities. The “X” letters denote the human eEF1A1 and eEF1A2 residues which are equivalent to the N329 and K333 residues involving actin binding of the yeast eEF1A molecule [13]. The threshold shows the arithmetic mean between two conservation scores whose sum of the sensitivity and specificity becomes maximum when the threshold is used as a cutoff value of the conservation score set.