Figure 3

Figure 3: Distribution of optimal values for E₀, k_cat and K_m obtained from multiple single stage optimizations.
Under these conditions, near-optimal H₂ production was obtained. Horizontal bars indicate average values. Top left shows results after optimization of E0 for all 13 enzymes; cellobiosephosphorylase (E1), phosphoglucomutase (E2),glucose-6-phosphate dehydrogenase (E3), 6-phosphogluconic dehydrogenase (E4), ribose 5-phosphate isomerase (E5),ribulose-5- phosphate 3-epimerase (E6), transketolase (E7), transaldolase (E8), triosephosphate isomerase (E9), aldolase (E10), fructose-1,6-bisphosphatase (E11), phosphoglucoseIsomerase (E12), NADP+dependent hydrogen dehydrogenase (E13). Top right shows results after optimization of kcat for all 14 reactions (see Method and Supplementary Table 1 for detailed description of the reactions); Bottom are the results after optimization of Km for all 19 substrates (1:Km_Pi of reaction r1; 2:Km_Cb of r1; 2:Km_Cb of r2; 3:Km_G1P of r2; 4:Km_G6P of r2; 5:Km_G6P of r3; 6:Km_NADP of r3; 7:Km_6PG of r4; 8:Km_NADP of r4; 9:Km_Ru5P of r5; 10:Km_Ru5P of r6; 11:Km_DHAP of r9; 12:Km_g3P of r9; 13:Km_DHAP of r10; 14:Km_g3P of r10; 15:Km_FdP of r11; 16:Km_F6P of r12; 17:Km_G6P of r12; 18:Km_NADP of r13; 19:Km_NADPH of r13).