Figure 3: The barcoding strategy used in 454 pyrosequencing
Each primer contains a region complementary to the 454 A or B adaptator sequencing primers, a variable barcode sequence length (in red) and a region complementary to ends of the 16S rRNA region targeted (Primer R or F)
Each primer contains a region complementary to the 454 A or B adaptator sequencing primers, a variable barcode sequence length (in red) and a region complementary to ends of the 16S rRNA region targeted (Primer R or F)