Figure 4: Effect of NaB on expression of RNA for E-cadherin and vimentin. Expression of RNA for E-cadherin and vimetin was detected by real-time RT-PCR. Expression levels were normalized to that of the control RNA (0 mM). Total RNAs were prepared from Ca9-22 (A), HSC-2 (B), -3 (C), and -4 (D) cells treated with 0, 0.3, 2.5 and 20 mM NaB for 4, 8, 12, and 24 h. Each bar represents the mean ± SD of three separate experiments in duplicate samples.