Cell Line
Percentage
Method
Reference
C6 (rat) 0.4 % Isolation of the SP fraction (Kondo et al., 2004)
C6 (rat) 4.21 % Nestin-labelled flow cytometry (Zhou et al., 2009)
C6 (rat) 86.5 % Tumor sphere culture,  isolation by CD133 marker, SP analysis (Shen et al., 2008)
C6 (rat) 100 % 1.52 Single cell isolation SP fraction (Zheng et al., 2007) (Qiang et al., 2009)
N29 (rat) 99.38 % 99.92 % CD133+ Cells Nestin-labelled flow cytometry (Bexell et al., 2009) (Bexell et al., 2009)
N32 (rat) 99.79 % 99.76 % CD133+ Cells Nestin-labelled flow cytometry (Bexell et al., 2009) (Bexell et al., 2009)
SK-MG-1 (human) 2.8 % Isolation of the SP fraction (Fukaya et al., 2010)
U87MG (human) 0.7 %, 1.34 % 0.9 % Isolation of the SP fraction Isolation of the SP fraction CD133+ Cells (Fukaya et al., 2010) (Chua et al., 2008) (Qiang et al., 2009)
U373MG (human) 0.4 %, 1.90 % Isolation of the SP fraction Isolation of the SP fraction (Fukaya et al., 2010) (Chua et al., 2008)
KNS42 (human) 0.5 % Isolation of the SP fraction (Fukaya et al., 2010)
U251 (human)
    1. %
0.63 % 3.2 % 2.05 %
Isolation of the SP fraction Isolation of the SP fraction CD133+ Cells Isolation of the SP fraction (Fukaya et al., 2010) (Qiang et al., 2009) (Qiang et al., 2009) (Chua et al., 2008)
T98 2.01 % Isolation of the SP fraction (Chua et al., 2008)
A172 6.81 % 2.1 % Isolation of the SP fraction CD133+ Cells (Chua et al., 2008) (Qiang et al., 2009)
Table 1: Detection of GSCs in established glioma cell lines.